Effect of Oocyte Retrieval Techniques on Yield and Quality of Caprine Oocytes
نویسندگان
چکیده
The efficiency of three oocyte collection methods from goat ovaries was assessed. Oocytes were collected by aspiration (n=165), slicing (n=213) and puncture (n=172). The mean yield of oocytes per ovary was found to be 3.93 ± 0.11, 4.44 ± 0.06 and 3.59 ± 0.07 by aspiration, slicing and puncture respectively. Slicing yielded significantly higher number of oocytes per ovary. Percentage yield of culturable quality oocytes (A, B and C) by different retrieval methods were 78.18, 76.05 and 81.39 respectively by aspiration, slicing and puncture. It was found that puncture technique yielded significantly higher culturable quality oocytes than aspiration and slicing.
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